Immobilized 2-(4-Hydrazinocarbonylphenyl)-4,5-diphenyl-imidazole as Solid Phase Luminophore in Peroxyoxalate Chemiluminescence

Einar PontÚn, Patrik Appelblad, Malin Stigbrand and Knut Irgum*
Department of Analytical Chemistry, Umeň University, S-901 87 Umeň, Sweden.

Kenichiro Nakashima
School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-Machi, Nagasaki 852, Japan.

A new luminophore for application in peroxyoxalate chemiluminescence is presented. An analogue of the well-known chemiluminescence compound lophine, namely 2-(4-hydrazinocarbonylphenyl)-4,5-diphenylimidazole (HCPI) has been covalently immobilized to controlled pore glass and a porous methacrylate resin. By using this reagent in a solid phase detection reactor, sensitive determinations of hydrogen peroxide have been demonstrated. In homogeneous solution HCPI emits poorly as a result of 1,1’-oxalyldiimidazole excitation, but when immobilized, its efficiency is almost comparable to highly efficient luminophores such as 3-aminofluoranthene. Linearity extends in the single stream flow system over several orders of magnitude with both two materials. The limit of detection was 1 nM (10 fmole injected), when using the porous methacrylate support.

Fresenius' J. Anal. Chem., 1996, 356, 84-89.

Last updated September 8, 1996 by Knut Irgum, e-mail

Since 96-09-23