Umeå UniversityUmeň University - Chemistry Courses in English

Chromatography D, 10 p

Topic and Background

Chromatographic techniques are of great importance in analytical chemistry, both for sample preparation and the separation of sample components in complex matrices. In recent years, the development of new methods for various types of sample components has been extremely rapid.

Aim of the Course

After having passed the course, the student should be capable of selecting the most suitable sample clean-up, separation, and detection method for a given analytical separation problem, as well as to implement, validate, and document this method in a practical application.

Course Contents

The initial theoretical part of the course deals with the thermodynamic, kinetic, and flow dynamic prerequisites for successful analytical separations using various forms of chromatography and differential migration techniques.

This section is followed by a treatment of the different forms of analytical gas and liquid chromatography (including supercritical chromatography), analytical electrophoretic methods, and membrane-based separation methods for analytical purposes. Sample preparation by the aid of various physical and chemical separation principles are also taught in this part of the course.

Special emphasis is put on aspects that enable the choice of "correct" separation and detection techniques for a particular analytical problem. Much attention is paid to detection, since sensitive and selective analytical methods are dependent on the possibilities for detecting and positively identifying the separated compounds. Chemical methods for separation- and detection-oriented derivatization are also dealt with in this section. The theoretical part of the course ends with a discussion of the working procedures for validation of developed methods, with examples from the pharmaceutical industry.

Among the separation methods trained in the laboratory part of the course are:

  • Capillary electrophoresis
  • Capillary gas chromatography with various injection and detection techniques
  • High performance liquid chromatography
  • Ion chromatography
  • Detection in liquid chromatography
    UV-VIS Spectroscopic, diode-array, and fluorescent and chemiluminescent detection
Within all the separative laborations there are sections that demonstrate the particular sample preparation, derivatization, and detection techniques that are necessary to enable analysis of real samples with satisfactory result.

Mini Project

The final laborative part of the course takes the form of a mandatory, practically oriented mini-project. This project is based on a separation problem that the student, after discussions with the course leader at the very start of the course, has decided to solve in a small group.

During the course, the students should actively search for applicable methods in the scientific literature, as well as make all necessary practical arrangements to ascertain that equipment and consumables are available when the terminal mini-project commences. Approximately half-ways through the course, the students will therefore present for eachother in a seminar what techniques they have chosen to solve the problem.

During the project period, which encompasses about a week of practical work, the group will try to solve the problem, and thereafter account for their results, partly in the form of a short seminar, but also in the form of a report, that should be designed as an analytical method.

Compulsory laboratory experiments are included in the course.

Course Dates 1998/99

  • November 2, 1998 to January 17, 1999. Full time course.
  • January 18 to March 21, 1999. Full time course.
Required Qualifications


  • Poole C.F and Poole S.K., "Chromatography Today", Elsevier: Amsterdam, 1991.
  • Additional material in the form of reprints, etc., available from the department at a nominal cost.
Other Advanced Level Courses in Analytical Chemistry and Chemometrics

For more information, contact the principal teacher of the course by clicking on the e-mail address, or by sending a traditional letter to:

Umeň University, Department of Chemistry, S-901 87 Umeň, Sweden.

You can also contact the Department of Chemistry by
Phone: int+46 90 7865238/7865173/7865262, or Fax: int+46 90 136310/167655

Department of Chemistry Home Page

This document was last updated on April 7, 1998.
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